PRKAB1 elisa kit product blogThe Bovine PRKAB1 prkab1 (Catalog #MBS288341) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS288341 ELISA Kit recognizes Bovine (General) PRKAB1.
The PRKAB1 prkab1 product has the following accession number(s) (GI #66792900) (NCBI Accession #NP_001019729.1) (Uniprot Accession #Q5BIS9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Bovine 5\'-AMP-activated protein kinase subunit beta-1 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AMPK subunit beta-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AMPK subunit beta-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AMPK subunit beta-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AMPK subunit beta-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Detection Range: 0.78 ng/ml -50.0 ng/ml. Bovine 5\'-AMP-activated protein kinase subunit beta-1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Acute Kidney Injury, Adenocarcinoma, Brain Diseases, Cardiovascular Diseases, Fatty Liver, Hypertrophy, Inflammation, Liver Diseases, Vascular Diseases, Weight Gain are some of the diseases may be linked to Bovine 5\'-AMP-activated protein kinase subunit beta-1 ELISA Kit. Kidney, Liver, Lung, Muscle, Ovary, Skin tissues are correlated with this protein. PRKAB1 also interacts with the following gene(s): PPARGC1A, PRKAA1, PRKAA2, PRKAG1, PRKAG2, PRKAG3, TSC2.