PGD2-MOX elisa kit product blogThe Porcine PGD2-MOX n/a (Catalog #MBS2511609) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2511609 ELISA Kit recognizes Porcine (General) PGD2-MOX.
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(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine PGD2-MOX concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with PGD2-MOX. During the reaction, PGD2-MOX in the sample or standard competes with a fixed amount of PGD2-MOX on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGD2-MOX. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PGD2-MOX in the samples is then determined by comparing the OD of the samples to the standard curve.
Samples: Serum, Plasma, Biological Fluids
Assay Type: Competitive
Detection Range: 31.25-2000pg/mL
Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL. Porcine PGD2-MOX (Prostaglandin D2-Methoxime) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.