OxiSelect Protein Carbamylation elisa kit product blogThe OxiSelect Protein Carbamylation n/a (Catalog #MBS168597) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the OxiSelect Protein Carbamylation Sandwich ELISA Kit with the following immunoassay(s):
Testing Data #2
Principle of the assay: CBL-BSA standards or protein samples are added to an Anti-CBL Antibody Coated Plate for 2 hours at 37 degree C. The CBL protein adducts present in the sample or standard are probed with an anti-CBL antibody, followed by an HRP conjugated secondary antibody. The protein CBL adduct content in an unknown sample is determined by comparing against a standard curve prepared from CBL-BSA standards.
Background: Carbamylation is a post-translational modification which occurs throughout the lifespan of proteins in vivo. Carbamylation results from the binding of isocyanic acid, spontaneously derived from high concentrations of urea and leading to the formation of carbamyl-lysine (CBL) (Figure 1). The carbamylation of proteins is usually associated with a partial or complete loss of protein function. It is known that elevated urea directly induces the formation of potentially atherogenic carbamylated LDL (cLDL). High blood concentrations of urea leading to the carbamylation process were detected in uremic patients and patients with end-stage renal disease. The OxiSelect Protein Carbamylation Sandwich ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of protein carbamylation. The quantity of carbamylated adduct in protein samples is determined by comparing its absorbance with that of a known CBL-BSA standard curve. The kit has a detection sensitivity limit of 1.5 ng/mL of CBL-BSA. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.
OxiSelect Protein Carbamylation Sandwich ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.