LHb elisa kit product blogThe Human LHb lhb (Catalog #MBS2033694) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2033694 ELISA Kit recognizes Human (General) LHb.
The LHb lhb product has the following accession number(s) (GI #119572817) (NCBI Accession #EAW52432.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Luteinizing Hormone Beta Polypeptide (LHb) ELISA Kit with the following immunoassay(s):
Typical Standard Curve/Testing Data
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Luteinizing Hormone Beta Polypeptide (LHb) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Luteinizing Hormone Beta Polypeptide (LHb) and unlabeled Luteinizing Hormone Beta Polypeptide (LHb) (Standards or samples) with the pre-coated antibody specific to Luteinizing Hormone Beta Polypeptide (LHb). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Luteinizing Hormone Beta Polypeptide (LHb) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Luteinizing Hormone Beta Polypeptide (LHb) in the sample.
Assay Type: Competitive Inhibition
Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range: 246.91-20000pg/mL
Sensitivity: < 90.4pg/mL. Application: Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Luteinizing Hormone Beta Polypeptide (LHb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Luteinizing Hormone Beta Polypeptide (LHb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =: SD/meanX100
Inter-Assay: CV<12%. Human Luteinizing Hormone Beta Polypeptide (LHb) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. LHb also interacts with the following gene(s): CGA, CYP17A1, EGR1, FOXO1, LHCGR, POMC. Brain, Embryonic Tissue, Pituitary Gland, Placenta tissues are correlated with this protein. The following patways have been known to be associated with this gene. Cardiovascular Diseases, Depressive Disorder, Heart Diseases, Hyperplasia, Hypogonadism, Infertility, Female, Inflammation, Myocardial Infarction, Nervous System Diseases, Pain are some of the diseases may be linked to Human Luteinizing Hormone Beta Polypeptide (LHb) ELISA Kit.