IL11 elisa kit product blogThe Mouse IL11 il11 (Catalog #MBS705108) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS705108 ELISA Kit recognizes Mouse (General) IL11.
The IL11 il11 product has the following accession number(s) (GI #6680393) (NCBI Accession #NP_032376.1) (Uniprot Accession #P47873). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Interleukin 11, IL-11 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Introduction: Interleukin 11 (IL-11) is a multifunctional cytokine first isolated in 1990 from bone marrow-derived stromal cells. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 is a member of the IL-6-type cytokine family, distinguished based on their use of a common transmembrane signal transducer. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. Besides from lymphopoietic/ hematopoietic and osteotrophic properties, it has functions in many tissues such as brain, gut and testis. IL-11 stimulates the growth of certain lymphocytes and, in the murine model, stimulates an increase in the cortical thickness and strength of long bones.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-11. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL-11 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3\',5,5\' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Serum, plasma, cell culture supernates, tissue homogenates
Detection Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 2 pg/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength: 450 nm
Sample Volume: 50-100ul.