IL-4 elisa kit product blogThe Canine IL-4 il4 (Catalog #MBS281566) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS281566 ELISA Kit recognizes Canine (General) IL-4.
The IL-4 il4 product has the following accession number(s) (GI #50978886) (NCBI Accession #NP_001003159.1) (Uniprot Accession #O77762). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL4 in samples. An antibody specific for IL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, Plasma, Other biological fluids
Assay Type: Sandwich. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Detection Wavelength: 450 nm. Canine Interleukin 4 (IL-4) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. IL-4 also interacts with the following gene(s): CSF2, IL13RA1, IL15, IL2RG, IL4R, IL5, IL6, NOS2. The following patways have been known to be associated with this gene. Asthma, Autoimmune Diseases, Cardiovascular Diseases, Hypersensitivity, Hypersensitivity, Immediate, Inflammation, Liver Diseases, Lung Diseases, Necrosis, Respiratory Hypersensitivity are some of the diseases may be linked to Canine Interleukin 4 (IL-4) ELISA Kit.