IKBalpha elisa kit product blogThe Rat IKBalpha n/a (Catalog #MBS2512400) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2512400 ELISA Kit recognizes Rat (General) IKBalpha.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat IKBalpha (Inhibitory Subunit of NF-kappaBalpha)ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IKBalpha in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IKBalpha. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat IKBalpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IKBalpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat IKBalpha. You can calculate the concentration of Rat IKBalpha in the samples by comparing the OD of the samples to the standard curve.
Samples: Serum, Plasma, Biological Fluids
Assay Type: Sandwich
Detection Range: 0.313-20ng/mL
Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL. Rat IKBalpha (Inhibitory Subunit of NF-kappaBalpha)ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.