Histamine elisa kit product blogThe Human Histamine n/a (Catalog #MBS494341) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS494341 ELISA Kit recognizes Human (General) Histamine.
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Intended Uses: Intended use and principle of the test Enzyme Immunoassay for the determination of Histamine in plasma and urine.
Principle of the Assay: In combination with the supplementary kit Histamine Release (Catalog # MBS494193, sold separately), the assay can be used for the determination of histamine release in heparinized whole blood. In the first part of the procedure, Histamine is acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated analyte concentrations in the standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-goat IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Determination of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations.
Background: Histamine belongs to the biogenic amines and is synthesized by decarboxylation from the amino acid histidine. It is synthesized by mast cells, basophils, platelets, histaminergic neurons, and enterochromaffine cells, where it is stored intracellularly in vesicles and released on stimulation. Histamine acts by binding to its 4 receptors (H1R, H2R, H3R and H4R) on target cells in various tissues. It causes smooth muscle cell contraction, vasodilatation, increased vascular permeability and mucus secretion, tachycardia, alterations of blood pressure, and arrhythmias. In humans, histamine is one of the most important mediators and takes part in the initial phase of an anaphylactic reaction ("immediate type" allergy). Of interest is also the determination of the histamine release from basophilic leucocytes in allergies.
Samples: Plasma and urine
Assay Type: Competitive. Histamine ELISA or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.