FT4 elisa kit product blogThe Chicken FT4 n/a (Catalog #MBS707279) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS707279 ELISA Kit recognizes Chicken (General) FT4.
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(Or you can also download the PDF Manual for complete product instructions).
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FT4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated FT4. A competitive inhibition reaction is launched between FT4 (Standards or samples) and Biotin-conjugated FT4 with the pre-coated antibody specific for FT4. The more amount of FT4 in samples, the less antibody bound by Biotin-conjugated FT4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of FT4 in the sample. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma.
Assay Type: Competitive
Detection Range: 4 pmol/l -64 pmol/l.
Sensitivity: 4 pmol/l. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess. Chicken free thyroxine (FT4) ELISA kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.