E1 / UBAE elisa kit product blogThe Canine E1 / UBAE uba5 (Catalog #MBS734536) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS734536 ELISA Kit recognizes Canine (General) E1 / UBAE.
The E1 / UBAE uba5 product has the following accession number(s) (GI #46389771) (NCBI Accession #BAD15375) (Uniprot Accession #Q9GZZ9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Canine E1/ubiquitin-activating enzyme ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: E1/UBAE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-E1/UBAE antibody and an E1/UBAE-HRP conjugate. The assay sample and buffer are incubated together with E1/UBAE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the E1/UBAE concentration since E1/UBAE from samples and E1/UBAE-HRP conjugate compete for the anti-E1/UBAE antibody binding site. Since the number of sites is limited, as more sites are occupied by E1/UBAE from the sample, fewer sites are left to bind E1/UBAE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The E1/UBAE concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0U/L. Intended Uses: This E1/UBAE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine E1/UBAE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Canine E1/ubiquitin-activating enzyme ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.