cGMP elisa kit product blogThe Deer cGMP n/a (Catalog #MBS282542) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS282542 ELISA Kit recognizes Deer (General) cGMP.
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Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate cGMP in samples. An antibody specific for cGMP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anycGMP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cGMP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cGMP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, Plasma, Other biological fluids
Assay Type: Sandwich. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Detection Wavelength: 450 nm. Deer Cyclic guanosine monophosphate (cGMP) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.