ANXA1 elisa kit product blogThe Bovine ANXA1 anxa1 (Catalog #MBS756020) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS756020 ELISA Kit recognizes Bovine (General) ANXA1.
The ANXA1 anxa1 product has the following accession number(s) (GI #4502101) (NCBI Accession #NP_000691.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Princple of the assay: ANXA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANXA1 antibody and an ANXA1-HRP conjugate. The assay sample and buffer are incubated together with ANXA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANXA1 concentration since ANXA1 from samples and ANXA1-HRP conjugate compete for the anti-ANXA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANXA1 from the sample, fewer sites are left to bind ANXA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANXA1 concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Intended Uses: This ANXA1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ANXA1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Bovine Annexin A1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Blood, Bone, Brain, Liver, Lung, Lymph Node, Muscle, Pituitary Gland, Stomach, Vascular tissues are correlated with this protein. ANXA1 also interacts with the following gene(s): FPR1, FPR2, FPR3, POMC, S100A11, S100A6. Brain Diseases, Carcinoma, Carcinoma, Squamous Cell, Cardiovascular Diseases, Disease Models, Animal, Inflammation, Lung Diseases, Necrosis, Neoplasm Metastasis, Pain are some of the diseases may be linked to Bovine Annexin A1 ELISA Kit.