anti TNF Alpha autoantibody elisa kit product blogThe Human anti TNF Alpha autoantibody n/a (Catalog #MBS7220931) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7220931 ELISA Kit recognizes Human (General) anti TNF Alpha autoantibody.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human anti TNF Alpha autoantibody ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: TNFalphaAb ELISA kit applies the competitive enzyme immunoassay technique utilizing TNFalpha antigen and an TNFalphaAb-HRP conjugate. The assay sample and buffer are incubated together with TNFalphaAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNFalphaAb concentration since TNFalphaAb from samples and TNFalphaAb-HRP conjugate compete for the TNFalpha antigen binding site. Since the number of sites is limited, as more sites are occupied by TNFalphaAb from the sample, fewer sites are left to bind TNFalphaAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNFalphaAb concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 ng/mL. Intended Uses: This TNFalphaAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TNFalphaAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Human anti TNF Alpha autoantibody ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.