ANG II R1-Ab elisa kit product blogThe Human ANG II R1-Ab n/a (Catalog #MBS762907) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS762907 ELISA Kit recognizes Human (General) ANG II R1-Ab.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Angiotensin II Receptor 1 Antibody ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. AT1AR was pre-coated onto 96-well plates. And the Biotin- labeled AT1AR was used as detection antigen. The standards, test samples and Biotin- labeled AT1AR were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin Conjugate was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ANG II R1-Ab amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ANG II R1-Ab can be calculated.
Samples: Serum, plasma, tissue homogenates and other biological fluids
Assay Type: Sandwich
Detection Range: 3.125-200ng/ml
Sensitivity: < 1.875ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ANG II R1-Ab were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ANG II R1-Ab were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%. Human Angiotensin II Receptor 1 Antibody ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.