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3-NT elisa kit product blog

Tags: ELISA Kit; Rat ELISA Kit; 3-Nitrotyrosine; 3-NT elisa kit; 3-NT;

     The Rat 3-NT n/a (Catalog #MBS284212) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS284212 ELISA Kit recognizes Rat (General) 3-NT.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate 3-NT in samples. An antibody specific for 3-NT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any3-NT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 3-NT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 3-NT bound in the initial step. The color development is stopped and the intensity of the color is measured.

3-NT n/a

Samples: Serum, Plasma, Other biological fluids
Assay Type: Sandwich. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength: 450 nm. Rat 3-nitrotyrosine (3-NT) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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