|The ZEN n/a (Catalog #MBS2548744) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase.
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Principle of the Assay: This kit uses Indirect-Competitive-ELISA as the method. It can detect Zearalenone (ZEN) in samples, such as grain, fodder, edible oil, beer, sauce and other samples. This kit is composed of Micro ELISA Plate, HRP conjugate, antibody, standard and other supplementary reagents. The micro-plate provided in this kit has been pre-coated with ZEN. During the reaction, ZEN in the samples or standard competes with ZEN on the solid phase supporter for sites of ZEN antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of ZEN. The concentration of ZEN in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Type: Competitive
Sensitivity: 0.03 ppb (ng/mL). ZEN (Zearalenone) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.