YPEL4 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; YPEL4; Protein yippee-like 4; YPEL4 elisa kit;
The Human YPEL4 ypel4 (Catalog #MBS283078) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS283078 ELISA Kit recognizes Human YPEL4.The YPEL4 ypel4 product has the following accession number(s) (GI #21450727) (NCBI Accession #NP_659445.1) (Uniprot Accession #Q96NS1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
YPEL4 gene contains 5 exons and spans about 5 kb. Exon 1 is untranslated. The mouse Ypel4 gene has the same structure.The deduced protein shares 99.2% amino acid identity with mouse Ypel4, 100% identity with monkey Ypel4, and 43.4% identity with Drosophila Yippee. YPEL4 shares 83.2 to 85.0% amino acid identity with YPEL1, YPEL2, and YPEL3. All YPEL proteins contain an 86-amino acid YPEL consensus sequence. PCR analysis detected YPEL4 expression in adult brain, lung, placenta, colon, ovary, small intestine, spleen, testis, and bone marrow, and in fetal brain, heart, liver, lung, and spleen. Immunofluorescent staining of COS-7 green monkey kidney cells localized Ypel4 to the centrosome and nucleolus during interphase and at several punctate structures around the mitotic apparatus during the mitotic phase.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate YPEL4 in samples. An antibody specific for YPEL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYPEL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YPEL4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YPEL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, Plasma, Other biological fluids
Assay Type: Sandwich. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength: 450 nm. Human Protein yippee-like 4 (YPEL4) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.