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XPF elisa kit product blog

Posted on 2016-04-18 20:58:02 by mybiosource_staff
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Tags: ELISA Kit; Sheep ELISA Kit; XPF; XPF elisa kit; Xeroderma Pigmentosum, Complementation Group F;
The Sheep XPF n/a (Catalog #MBS756197) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS756197 ELISA Kit recognizes Sheep (Ovine, General) XPF.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Principle of the assay: XPF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-XPF antibody and an XPF-HRP conjugate. The assay sample and buffer are incubated together with XPF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the XPF concentration since XPF from samples and XPF-HRP conjugate compete for the anti-XPF antibody binding site. Since the number of sites is limited, as more sites are occupied by XPF from the sample, fewer sites are left to bind XPF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The XPF concentration in each sample is interpolated from this standard curve.

XPF n/a

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 0.5-10ng/mL
Sensitivity: 0.1ng/mL. Sheep Xeroderma Pigmentosum, Complementation Group F ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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