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WT1 elisa kit product blog

Posted on 2017-06-03 05:28:12 by mybiosource_staff
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Tags: ELISA Kit; Mouse ELISA Kit; Wilms Tumor Protein; WT1 elisa kit; WT1;
The Mouse WT1 wt1 (Catalog #MBS280490) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS280490 ELISA Kit recognizes Mouse (Murine, General) WT1.

The WT1 wt1 product has the following accession number(s) (GI #139779) (NCBI Accession #P22561.1) (Uniprot Accession #P22561). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WT1 in samples. An antibody specific for WT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

WT1 wt1

Samples: Serum, Plasma, Other biological fluids
Assay Type: Sandwich. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength: 450 nm. Mouse Wilms tumor protein (WT1) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Adenocarcinoma, Carcinoma, Chromosome Aberrations, Denys-Drash Syndrome, Hematologic Diseases, Kidney Diseases, Leukemia, Myeloid, Leukemia, Myeloid, Acute, Neoplasms, Wilms Tumor are some of the diseases may be linked to Mouse Wilms tumor protein (WT1) ELISA Kit. Blood, Brain, Embryonic Tissue, Heart, Kidney, Lung, Ovary, Pancreas, Testis, Uterus tissues are correlated with this protein.
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