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VSIG2 elisa kit product blog

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Posted on 2015-12-01 01:54:19 by mybiosource_staff
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Tags: ELISA Kit; Human ELISA Kit; VSIG2; VSIG2 elisa kit; V-Set And Immunoglobulin Domain Containing Protein 2 (VSIG2);
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The Human VSIG2 vsig2 (Catalog #MBS2024244) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2024244 ELISA Kit recognizes Human (General) VSIG2.

The VSIG2 vsig2 product has the following accession number(s) (GI #21361429) (NCBI Accession #NP_055127.2) (Uniprot Accession #Q96IQ7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the V-Set And Immunoglobulin Domain Containing Protein 2 (VSIG2) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) VSIG2.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to V-Set And Immunoglobulin Domain Containing Protein 2 (VSIG2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled V-Set And Immunoglobulin Domain Containing Protein 2 (VSIG2) and unlabeled V-Set And Immunoglobulin Domain Containing Protein 2 (VSIG2) (Standards or samples) with the pre-coated antibody specific to V-Set And Immunoglobulin Domain Containing Protein 2 (VSIG2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of V-Set And Immunoglobulin Domain Containing Protein 2 (VSIG2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of V-Set And Immunoglobulin Domain Containing Protein 2 (VSIG2) in the sample.

Samples: Tissue homogenates and other biological fluids
Assay Type: Competitive
Detection Range: 493.83-40000pg/mL
Sensitivity: Typically less than 152.5pg/mL. Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Set And Immunoglobulin Domain Containing Protein 2 (VSIG2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Set And Immunoglobulin Domain Containing Protein 2 (VSIG2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Assay Procedure Summary: 1. Prepare all reagents, samples and standards;
2. Add 50uL standard or sample to each well.
    And then add 50uL prepared Detection Reagent An immediately.
    Shake and mix. Incubate 1 hour at 37 degree C;
3. Aspirate and wash 3 times;
4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
5. Aspirate and wash 5 times;
6. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
7. Add 50uL Stop Solution. Read at 450 nm immediately. V-Set And Immunoglobulin Domain Containing Protein 2 (VSIG2) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. VSIG2 also interacts with the following gene(s): LRRC20.
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