ViraDuctin Adenovirus transduction kit product blog
Tags: Transduction Kit; ViraDuctin Adenovirus; ViraDuctin Adenovirus transduction kit;
The ViraDuctin Adenovirus n/a (Catalog #MBS169079) is a Transduction Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given transduction kit. We\'ve tested the ViraDuctin Adenovirus Transduction Kit (50 Transductions) with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Background: Recombinant adenoviruses have tremendous potential in both research and therapeutic applications. There are numerous advantages in using an adenovirus to introduce genetic material into host cells. The virus has been used to infect many mammalian cell types (both replicative and non-replicative) for high expression of the recombinant protein. Recombinant adenoviruses are especially useful for gene transfer and protein expression in cell lines that have low transfection efficiency with liposome. After entering cells, the virus remains epichromosomal (i.e. does not integrate into the host chromosome so does not activate or inactivate host genes). Recently, recombinant adenoviruses have been used to deliver RNAi into cells. A limiting step in adenoviral uptake is the entry into target cells, which is mediated by the coxsackievirus-adenovirus receptor (CAR) and cellular alphavbeta integrins. In many cell types, these receptors are either absent or expressed at low levels, leading to poor transduction efficiency by adenovirus. For instance, most stem cells and fibroblast-type cells are poorly infected by adenoviral vectors because of low expression of CAR.