VIM elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; Vimentin; VIM elisa kit; VIM;
The Human VIM vim (Catalog #MBS494838) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS494838 ELISA Kit recognizes Human VIM.The VIM vim product has the following accession number(s) (GI #62414289) (NCBI Accession #NP_003371.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Vimentin ELISA with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: For detection of VEGFR2 in human serum, plasma, urine, tissue samples or cell culture supernates. For research use only.
Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-VEGFR2 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-VEGFR2 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the VEGFR2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of VEGFR2 can be calculated
Background/Introduction: VEGF receptors are receptors for vascular endothelial growth factor (VEGF). They play an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis.There are three main subtypes of VEGFR, numbered 1, 2 and 3. VEGFR2, also known as Kinase insert domain receptor (KDR) or Flk1 (Fetal Liver Kinase 1), plays an important role as negative regulator of VEGFA-and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Furthermore, therapeutic inhibition of VEGFR-2 action is now having an impact in the clinic for the treatment of a number of diseases.
Samples: Human Serum, Plasma, Urine, Tissue Samples Or Cell Culture Supernates
Assay Type: Sandwich
Detection Range: 30pg/ml-800pg/ml
Sensitivity: <10pg/ml. Vimentin ELISA or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Breast Neoplasms, Disease Models, Animal, Hemorrhage, Inflammation, Kidney Diseases, Liver Diseases, Neoplasm Metastasis, Nervous System Diseases, Neurodegenerative Diseases, Pain are some of the diseases may be linked to Vimentin ELISA. Blood, Bone, Bone Marrow, Brain, Eye, Ganglia, Kidney, Liver, Muscle, Nerve tissues are correlated with this protein.