Urokinase assay kit product blog
Tags: Urokinase; Assay Kit; Urokinase assay kit;
The Urokinase n/a (Catalog #MBS841840) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the Urokinase Activity Fluorometric Assay Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Product Information: Urokinase Activity Fluorometric Assay Kit: The most sensitive kit on the market to measure urokinase activity in cell/nuclear extracts & serum. 100 Assays.
Background: Urokinase (urokinase-type plasminogen activator or uPA, EC 3.4.21.73) is a serine protease, which catalyzes the conversion of the plasminogen to active plasmin, which in turn participates in thrombolysis. MyBioSource\'s Urokinase Fluorimetric Activity Assay Kit utilizes an AMC-based peptide substrate containing the recognition sequence for Urokinase. Urokinase present in the sample catalyzes the cleavage of the substrate and releases AMC, which can be easily quantified by measuring its fluorescence at Ex/Em = 350/450 nm. This sensitive assay detects very low levels of Urokinase activity in biological samples (as low as 0.02 IU/ml).
Summary: Detection method- Fluorescence (Ex/Em = 350/450 nm)
Application: - Measuring Urokinase activity of purified enzymes - Measuring Urokinase activity in serum & cell/nuclear extracts.
Samples: Cell & nuclear extract
Serum. Features & Benefits: Simple & convenient procedure.
Can detect urokinasse activity as low as 0.02 IU/ml.
Kit contains all necessary reagents for measuring urokinase activity.