Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow UPA elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • DSP elisa kit blog
 • CHRNA5 elisa kit blog
 • FcepsilonRIalpha/beta elisa kit blog
 • TAC4 elisa kit blog
 • SRP72 elisa kit blog
 • UTRN elisa kit blog
 • FcepsilonRI elisa kit blog
 • C6orf127 elisa kit blog
 • POT1 elisa kit blog
 • HBCO elisa kit blog
 • EGR3 elisa kit blog
 • TNFRSF1A elisa kit blog
 • IL-1ra elisa kit blog
 • FP elisa kit blog
 • MART/MELAN-A elisa kit blog
 • TGL elisa kit blog
 • C16orf90 elisa kit blog
 • HSP27 elisa kit blog
 • EIF6 elisa kit blog
 • XDH elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • MAML1 - Mastermind Peptide
 • IGFBP2 Antibody
 • lamin B receptor (LBR) Antibody
 • CALD ELISA Kit
 • C-C chemokine receptor type 7 (Ccr7) Recombinant Protein
 • E3 ubiquitin-protein ligase ZFP91 (ZFP91) Recombinant Protein
 • ALG1 cDNA Clone
 • Hypoxia Up Regulated 1 (HYOU1) ELISA Kit
 • Thyroid Hormone Receptor Beta (THRb) ELISA Kit
 • GHRH ELISA Kit
 • L-lactate dehydrogenase A chain (LDHA) Recombinant Protein
 • ubiquitin specific peptidase 24 (USP24) ELISA Kit
 • IL-21 ELISA Kit
 • Programmed Cell Death Protein 1 (PDCD1LG1) Recombinant Protein
 downarrow more ...
separator

UPA elisa kit product blog

separator
Posted on 2015-09-09 00:53:19 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Porcine ELISA Kit; uPA; uPA elisa kit; Urokinase plasminogen activator;
separator
The Porcine UPA plau (Catalog #MBS741889) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS741889 ELISA Kit recognizes Porcine (Pig, Swine, General) UPA.

The UPA plau product has the following accession number(s) (GI #56068043) (NCBI Accession #AAV70488.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Porcine Urokinase plasminogen activator ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) UPA.

Principle of the Assay: UPA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-UPA antibody and an UPA-HRP conjugate. The assay sample and buffer are incubated together with UPA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UPA concentration since UPA from samples and UPA-HRP conjugate compete for the anti-UPA antibody binding site. Since the number of sites is limited, as more sites are occupied by UPA from the sample, fewer sites are left to bind UPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UPA concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 pg/mL. Intended Uses: This UPA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine UPA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Porcine Urokinase plasminogen activator ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. UPA also interacts with the following gene(s): EGFR, FN1, MMP9, PLAUR, PLG, SERPINB2, SERPINE1, VLDLR. The following patways have been known to be associated with this gene.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions