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UCP-1 clia kit product blog

Posted on 2016-01-15 03:44:32 by mybiosource_staff
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Tags: CLIA Kit; UCP-1; Mouse CLIA Kit; UCP-1 (Uncoupling Protein 1, Mitochondrial); UCP-1 clia kit;
The Mouse UCP-1 n/a (Catalog #MBS2531392) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2531392 CLIA Kit recognizes Mouse (Murine, General) UCP-1.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given clia kit. We\'ve tested the Mouse UCP-1 (Uncoupling Protein 1, Mitochondrial) CLIA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) UCP-1.

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of UCP-1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to UCP-1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for UCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain UCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of UCP-1. You can calculate the concentration of UCP-1 in the samples by comparing the RLU value of the samples to the standard curve.

Assay Type: Sandwich
Detection Range: 31.25-2000pg/mL
Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL.
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