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TRH elisa kit product blog

Posted on 2017-03-30 16:56:10 by mybiosource_staff
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Tags: ELISA Kit; Mouse ELISA Kit; TRH elisa kit; TRH; Thyrotropin Releasing Hormone (TRH);
The Mouse TRH trh (Catalog #MBS2088148) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2088148 ELISA Kit recognizes Mouse (Murine, General) TRH.

The TRH trh product has the following accession number(s) (GI #6005920) (NCBI Accession #NP_009048.1) (Uniprot Accession #P20396). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyrotropin Releasing Hormone (TRH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyrotropin Releasing Hormone (TRH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyrotropin Releasing Hormone (TRH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyrotropin Releasing Hormone (TRH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

TRH trh

Assay Type: Double-antibody Sandwich. Application: Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyrotropin Releasing Hormone (TRH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyrotropin Releasing Hormone (TRH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =: SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%. Thyrotropin Releasing Hormone (TRH) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Bipolar Disorder, Brain Diseases, Disease Models, Animal, Drug Toxicity, Hypertension, Hypothermia, Nervous System Diseases, Pain, Seizures, THYROTROPIN-RELEASING HORMONE DEFICIENCY are some of the diseases may be linked to Thyrotropin Releasing Hormone (TRH) ELISA Kit. The following patways have been known to be associated with this gene. Bone Marrow, Brain, Cervix, Eye, Lung, Ovary, Pineal Gland, Uncharacterized Tissue tissues are correlated with this protein. TRH also interacts with the following gene(s): CCK, GCG, GHRH, GNRH1, GRP, NTS, PRL, TRHR.
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