Total Carbohydrate assay kit product blog
Tags: Assay Kit; Total Carbohydrate; Total Carbohydrate assay kit;
The Total Carbohydrate n/a (Catalog #MBS169243) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the Total Carbohydrate Assay Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: Total Carbohydrate Assay Kit measures total carbohydrate within food samples, urine, serum, plasma, lysate, or tissue samples based on the phenol-sulfuric acid method. Carbohydrates are hydrolyzed to furfural and other derivative forms in the presence of sulfuric acid. Upon addition of Developing Solution, a chromagen is formed that can be detected at 490 nm (Figure 1).
Background/Introduction: Carbohydrates are molecules made up of oxygen, hydrogen, and carbon. Typically carbohydrates contain a hydrogen:oxygen atom ratio of 2:1 (as with water). In the field of biochemistry, carbohydrates are viewed synonymously with saccharides, which describe sugars, starch, and cellulose. The saccharides can be further categorized as monosaccharides, disaccharides, oligosaccharides, and polysaccharides. Typically the lower molecular weight monosaccharides (such as glucose) and disaccharides (such as sucrose) are referred to as sugars. Carbohydrates perform key roles in living organisms such as storing energy, as parts of coenzymes, as part of genetic material (DNA and RNA) as well as structural roles. The measurement of total carbohydrate concentration is important in several fields such as the food industry, pharmaceutical research, petroleum industry, as well as environmental research. Many techniques such as light scattering, nuclear magnetic resonance, capillary electrophoresis, infrared spectroscopy, and chromatography have been used to detect total carbohydrates, but these techniques are costly, time consuming, and require complex analytical skills. Total Carbohydrate Assay Kit is a simple colorimetric assay that measures the amount of total carbohydrate present in foods, urine, plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and unknown samples. Sample carbohydrate concentrations are determined by comparison with a known glucose standard. The kit has a detection sensitivity limit of 62.5 uM glucose.