Total Bile Acid assay kit product blog
Tags: Assay Kit; Total Bile Acid; Total Bile Acid assay kit;
The Total Bile Acid n/a (Catalog #MBS168476) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the Total Bile Acid Assay Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The Total Bile Acid Assay Kit measures the total bile acid within serum, plasma, and cell or tissue lysate samples. The assay is based on an enzyme driven reaction: when bile acids are incubated in the presence of 3alpha-hydroxysteroid dehydrogenase (3alpha-HSD), NADH, and thio-NAD+, thio-NAD+ is converted to its reduced form Thio-NADH. Thio-NADH is then detected colorimetrically as an absorbance increase at 405 nm (Figure 1).
Background/Introduction: Bile is a complex mixture of lipids, protein, carbohydrates, mineral salts, vitamins, and various trace elements, with bile acids making up about 67% of the total composition. Bile acids are produced from excess cholesterol, secreted from the liver, absorbed into the small intestines, and returned to the liver with portal blood. While bile acid synthesis is critical for the removal of cholesterol from the body, bile acids are also needed for proper uptake of dietary lipids, fat soluble vitamins, and other nutrients into the small intestines. Under physiological conditions, newly synthesized bile acids are conjugated to glycine or taurine to form bile salts, and not much free bile acid is actually found in bile. Determining circulatory levels of bile acids can be used to identify or identify certain liver diseases. In addition, elevated serum bile levels have been observed in intrahepatic cholestasis of pregnancy cases. The Total Bile Acid Assay Kit is a simple colorimetric assay that measures the amount of total bile acid present in plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, bile acid standards and unknown samples. Sample bile acid concentrations are determined by comparison with a known bile acid standard.