Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow TGFbeta2 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • HADHA elisa kit blog
 • ACBD6 elisa kit blog
 • ADAMTSL2 elisa kit blog
 • FNBP1 elisa kit blog
 • Slit2 elisa kit blog
 • LLGL1 elisa kit blog
 • C1S elisa kit blog
 • sCD21 elisa kit blog
 • Il4r elisa kit blog
 • ECHS1 elisa kit blog
 • NEO elisa kit blog
 • TSPAN12 elisa kit blog
 • FNDC3B elisa kit blog
 • TPP elisa kit blog
 • TYRO3 elisa kit blog
 • LIPC elisa kit blog
 • SLC26A3 elisa kit blog
 • IL-22 elisa kit blog
 • RIMS1 elisa kit blog
 • FMO1 elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • FUNDC1 cDNA Clone
 • Procalcitonin Antibody
 • Glycated Hemoglobin A1c (HbA1c) ELISA Kit
 • Lamin B1 (LMNB1) Antibody
 • Protease Inhibitor Cocktail (Multipurpose) Inhibitor
 • small nuclear ribonucleoprotein, snRNP-Sm antibody ELISA Kit
 • PSD95 (DLG4) Antibody
 • S-phase Kinase-associated Protein 2 (Skp2) Antibody
 • Tumor Necrosis Factor-alpha (TNF) Recombinant Protein
 • S Antigen (SAG) ELISA Kit
 • Adiponectin (Acrp30) ELISA Kit
 • SH3BGRL3 Antibody
 downarrow more ...

TGFbeta2 elisa kit product blog

Posted on 2015-09-11 16:25:44 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Canine ELISA Kit; Transforming Growth Factor beta2; TGFbeta2 elisa kit; TGFbeta2;
The Canine TGFbeta2 tgfb2 (Catalog #MBS737118) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS737118 ELISA Kit recognizes Canine (Dog, General) TGFbeta2.

The TGFbeta2 tgfb2 product has the following accession number(s) (GI #208022653) (NCBI Accession #NP_001129071.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Canine Transforming Growth Factor beta2 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) TGFbeta2.

Principle of the assay: TGF?2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TGF?2 antibody and an TGF?2-HRP conjugate. The assay sample and buffer are incubated together with TGF?2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TGF?2 concentration since TGF?2 from samples and TGF?2-HRP conjugate compete for the anti-TGF?2 antibody binding site. Since the number of sites is limited, as more sites are occupied by TGF?2 from the sample, fewer sites are left to bind TGF?2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGF?2 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Sandwich
Detection Range: 50-1000pg/mL
Sensitivity: 1.0pg/mL. Intended Uses: This TGF?2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine TGF?2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Canine Transforming Growth Factor beta2 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Bone, Brain, Connective Tissue, Embryonic Tissue, Eye, Heart, Lung, Muscle, Skin, Vascular tissues are correlated with this protein.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions