There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%. TGFB1 tgfb1 (Catalog #MBS1751056) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS1751056 ELISA Kit recognizes Bovine
There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%. TGFB1. MyBioSource\'s TGF Beta 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the TGFB1 tgfb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TGFB1 tgfb1 product has the following accession number(s) (GI #63025222) (NCBI Accession #NP_000651.3) (Uniprot Accession #P01137). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Bovine TGF Beta 1 PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: Bovine TGF? 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF? 1 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO, Immunogen sequence: A279-S390) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF? 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Bovine TGF? 1 amount of sample captured in plate.
Background: Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion.TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein.The standard product used in this kit is recombinant TGF? 1 with the molecular mass of 25KDa.
Samples: cell culture supernates, serum, plasma (EDTA) and urine
Detection Range: 15.6pg/ml-1000pg/ml
Sensitivity: < 1pg/ml. Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Bovine TGF Beta 1 PicoKine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Blood, Bone, Bone Marrow, Connective Tissue, Heart, Kidney, Liver, Lung, Muscle, Skin tissues are correlated with this protein. TGFB1 also interacts with the following gene(s): BMP2, CTGF, DCN, ENG, MMP2, SPARC, TGFB2, TGFBR2, TGFBR3, THBS1. Asthma, Diabetes Mellitus, Experimental, Diabetic Nephropathies, Fibrosis, Hypersensitivity, Hypertension, Liver Cirrhosis, Lung Diseases, Proteinuria, Pulmonary Fibrosis are some of the diseases may be linked to Bovine TGF Beta 1 PicoKine ELISA Kit.