|The Mouse TGF-beta1 tgfb1 (Catalog #MBS843318) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS843318 ELISA Kit recognizes Mouse (Murine, General) TGF-beta1.
Application- Quantitative protein detection, establishing normal range. Researchers should empirically determine the suitability of the TGF-beta1 tgfb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TGF-beta1 tgfb1 product has the following accession number(s) (GI #6755775) (NCBI Accession #NP_035707.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the TGF-beta1 (mouse) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Product Information: TGF-beta 1 (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of mouse TGF-beta 1 in serum, plasma, cell culture supernatants & urine. Detection Range: 15.6 pg/ml - 1000 pg/ml. 100 assays.
Background: TGF-beta1 is a multifunctional cytokine involved in cell growth, differentiation and inflammatory pathways. MyBioSource\'s mouse TGF-beta1 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rat monoclonal antibody specific for mouse TGF-beta1 is coated on a 96-well plate. Standards and test samples are added to the wells and TGF-beta1 present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for TGF-beta1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse TGF-beta1 captured onto the plate. This ELISA kit shows <1% cross-reactivity with TGF-beta2, TGF-beta3 and TGF-beta5. Detection Range: 15.6 pg/ml - 1000 pg/ml. Sensitivity: < 2 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Application- Quantitative protein detection, establishing normal range etc.
Samples: Serum & plasma (EDTA), Cell Culture Supernatants, urine
Assay Type: Sandwich. Detection Method: Absorbance (450 nm)
Features & Benefits: Easy, convenient and reliable method to assay for mouse TGF-beta 1.
Detection Range: 15.6-1000 pg/ml. Sensitivity: < 2 pg/ml.
This ELISA kit shows <1% cross-reactivity with TGFbeta2, TGFbeta3 & TGFbeta5. TGF-beta1 (mouse) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Asthma, Diabetes Mellitus, Experimental, Diabetic Nephropathies, Fibrosis, Hypersensitivity, Hypertension, Liver Cirrhosis, Lung Diseases, Proteinuria, Pulmonary Fibrosis are some of the diseases may be linked to TGF-beta1 (mouse) ELISA Kit. The following patways have been known to be associated with this gene. Bone Marrow, Embryonic Tissue, Eye, Intestine, Joint, Liver, Lung, Muscle, Skin, Spleen tissues are correlated with this protein.