|The Rat TGF-b1 tgfb1 (Catalog #MBS2531114) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2531114 CLIA Kit recognizes Rat (Rattus, General) TGF-b1.
The TGF-b1 tgfb1 product has the following accession number(s) (GI #63025222) (NCBI Accession #NP_000651.3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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for complete product instructions).
Please refer to the product datasheet for known applications of a given clia kit. We\'ve tested the Rat TGF-beta1 (Transforming Growth Factor beta1) CLIA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of TGF-beta1 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to TGF-beta1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TGF-beta1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TGF-beta1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of TGF-beta1. You can calculate the concentration of TGF-beta1 in the samples by comparing the RLU value of the samples to the standard curve.
Assay Type: Sandwich
Detection Range: 12.5-800pg/mL
Sensitivity: Min: 7.5pg/mL; Max: 800pg/mL. Blood, Bone, Bone Marrow, Connective Tissue, Heart, Kidney, Liver, Lung, Muscle, Skin tissues are correlated with this protein. TGF-b1 also interacts with the following gene(s): ACVRL1, APP, DCN, LTBP1, LTBP4, SMAD2, SMAD3, SMAD7, TGFBR1, TGFBR2. The following patways have been known to be associated with this gene.