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Testosterone elisa kit product blog

Posted on 2018-03-24 07:52:54 by mybiosource_staff
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Tags: ELISA Kit; Rat ELISA Kit; Testosterone; Testosterone elisa kit;
The Rat Testosterone n/a (Catalog #MBS9424769) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS9424769 ELISA Kit recognizes Rat (Rattus, General) Testosterone.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbtt antibody. Standards or samples are added to the appropliate microtiter plate wells with an antibody specific for testosterone and Horseradish Peroxidase (HRP) conjugated testosterone. The competitive inhibition reaction is launched between with HRP labeled testosterone and unlabeled testosterone with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of testosterone in the sample. The color development is stopped and the intensity of the color is measured.

Testosterone n/a

Samples: serum, plasma, cell culture supernates, tissue homogenates
Assay Type: Quantitative Competitive
Detection Range: 0.13 ng/ml-25.6 ng/ml
Sensitivity: <0.06 ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess. Rat Testosterone, T ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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