TDO elisa kit product blog
Tags: ELISA Kit; TDO elisa kit; TDO; Guinea Pig ELISA Kit; Tryptophan-2, 3-dioxygenase;
The Guinea Pig TDO tdo2 (Catalog #MBS750321) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS750321 ELISA Kit recognizes Guinea Pig TDO.The TDO tdo2 product has the following accession number(s) (GI #5032165) (NCBI Accession #NP_005642.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: This TDO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig TDO. This ELISA kit for research use only!
Principle of the Assay: TDO ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TDO antibody and an TDO-HRP conjugate. The assay sample and buffer are incubated together with TDO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TDO concentration since TDO from samples and TDO-HRP conjugate compete for the anti-TDO antibody binding site. Since the number of sites is limited, as more sites are occupied by TDO from the sample, fewer sites are left to bind TDO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TDO concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 1.0-25ng/mL
Sensitivity: 0.1ng/mL. Guinea pig Tryptophan-2, 3-dioxygenase ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Brain Diseases, Disease Models, Animal, Inflammation, Kidney Diseases, Learning Disorders, Liver Diseases, Lung Neoplasms, Memory Disorders, Nervous System Diseases, Weight Loss are some of the diseases may be linked to Guinea pig Tryptophan-2, 3-dioxygenase ELISA Kit. Blood, Brain, Connective Tissue, Embryonic Tissue, Heart, Liver, Lung, Testis tissues are correlated with this protein. The following patways have been known to be associated with this gene.