TDO elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; TDO elisa kit; TDO; Tryptophan-2, 3-dioxygenase;
The Mouse TDO tdo2 (Catalog #MBS722857) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS722857 ELISA Kit recognizes Mouse TDO.The TDO tdo2 product has the following accession number(s) (GI #5032165) (NCBI Accession #NP_005642.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Tryptophan-2, 3-dioxygenase ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This TDO2,3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of MouseTDO2,3. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: TDO2,3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TDO2,3 antibody and an TDO2,3-HRP conjugate. The assay sample and buffer are incubated together with TDO2,3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TDO2,3 concentration since TDO2,3 from samples and TDO2,3-HRP conjugate compete for the anti-TDO2,3 antibody binding site. Since the number of sites is limited, as more sites are occupied by TDO2,3 from the sample, fewer sites are left to bind TDO2,3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TDO2,3 concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 0.1 ng/mL. Mouse Tryptophan-2, 3-dioxygenase ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Brain Diseases, Disease Models, Animal, Inflammation, Kidney Diseases, Learning Disorders, Liver Diseases, Lung Neoplasms, Memory Disorders, Nervous System Diseases, Weight Loss are some of the diseases may be linked to Mouse Tryptophan-2, 3-dioxygenase ELISA Kit.