StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible) assay kit product blog
Tags: Assay Kit; StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible); StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible) assay kit;
The StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible) n/a (Catalog #MBS168338) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible) with the following immunoassay(s):
Testing Data
Testing Data #2
Background: Several recent studies have revealed that stem cells from various types of self-renewing tissue could be cultured in suspension. For example, neuronal stem cells grow as neurospheres that do not depend on a particular substrate. Similarly, mammary stem cells or stem cells from skin can also grow in suspension using similar culture conditions. These cells differentiate into various cell lineages after exposure to extracellular matrix molecule (ECM) or serum. Additionally, various cancer stem cells have been successfully grown under these conditions. These results demonstrate that stem cells can survive and proliferate in anchorage-independent culture model, and clonogenic activity is a sensitive indicator of undifferentiated stem cells. Traditionally, the colony formation assay is a common method to monitor anchorage-independent growth, which measures proliferation in a semisolid culture media after 3-4 weeks by manual counting of colonies. Standard assays are usually performed in 100-mm or 60 mm dishes, where cells are allowed to grow inside a semisolid culture media for 3-4 weeks before sizable colonies appear (> 40 cells/colony). This method is quite cumbersome, time-consuming, and difficult when testing a large number of samples. Additionally, the manual counting of colonies is highly subjective; with varying colony sizes, it\'s difficult to determine meaningful results. StemTag 96-Well Stem Cell Colony Formation Assay does not involve subjective manual counting of colonies or require a 3-4 week incubation period. Instead cells are incubated only 6-8 days in a proprietary semisolid agar media before being solubilized, lysed and detected by the patented CyQuant GR Dye in a fluorescence plate reader or assayed for alkaline phosphatase activity colorimetrically (Figure 1). Alternatively, viable colony-forming cells (CFCs) can be easily recovered for further culturing and testing. This format provides a quantitative, high-throughput method to accurately measure colony formation, while the short incubation time makes it possible to assay transiently transfected cells. The StemTag 96-Well Stem Cell Colony Formation Assay provides a stringent, anchorage-independent model for measuring stem cell colony formation, and allows for cell recovery. Each kit provides sufficient quantities to perform 96 tests in a microtiter plate. The assay may be modified for use in other plate formats (see Table 1 under Assay Protocol).