Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow StemTAG Alkaline Phosphatase assay kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • alphaENOL elisa kit blog
 • SLC22A16 elisa kit blog
 • Ischemia Modified Albumin elisa kit blog
 • DSDNAAB elisa kit blog
 • JKAMP elisa kit blog
 • BMP3 elisa kit blog
 • Cardiolipin IgG/IgM elisa kit blog
 • TUBGCP5 elisa kit blog
 • SLC10A7 elisa kit blog
 • HM13 elisa kit blog
 • Fibrin elisa kit blog
 • CCDC27 elisa kit blog
 • SEPT12 elisa kit blog
 • KiSS-1 receptor elisa kit blog
 • DSPP elisa kit blog
 • HMW ADNP elisa kit blog
 • SMYD3 elisa kit blog
 • OR51F2 elisa kit blog
 • QRFP elisa kit blog
 • EIF5B elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • PEK-PERK (Ab-981) Antibody
 • Serum Albumin (SA) ELISA Kit
 • Granulocyte Colony Stimulating Factor (GCSF) ELISA Kit
 • Glycosylated Serum Protein (GSP) ELISA Kit
 • Oncostatin M (OSM) ELISA Kit
 • Stromal Cell Derived Factor 1 (SDF1) ELISA Kit
 • Stathmin 1 (STMN1) ELISA Kit
 • Contactin 3 (CNTN3) ELISA Kit
 • Bone Morphogenetic Protein 15 (BMP15) ELISA Kit
 • Insulin Like Growth Factor1 (IGF1) ELISA Kit
 • Runt Related Transcription Factor 2 (RUNX2) ELISA Kit
 • Acyl Ghrelin (A-GHR) ELISA Kit
 • Stromal Cell Derived Factor 4 (SDF4) ELISA Kit
 • Endometrium Antibody (EMAB) ELISA Kit
 downarrow more ...

StemTAG Alkaline Phosphatase assay kit product blog

Posted on 2017-03-04 02:42:06 by mybiosource_staff
Share it on ...     
Tags: Assay Kit; StemTAG Alkaline Phosphatase; StemTAG Alkaline Phosphatase assay kit;
The StemTAG Alkaline Phosphatase n/a (Catalog #MBS169143) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the StemTAG Alkaline Phosphatase (Staining and Activity Assay Kit, Fluorometric) with the following immunoassay(s):
Testing Data
Testing Data StemTAG Alkaline Phosphatase.

Testing Data #2
Testing Data #2 StemTAG Alkaline Phosphatase.

Testing Data #3
Testing Data #3 StemTAG Alkaline Phosphatase.

Background: Embryonic stem (ES) cells are continuous proliferating stem cell lines of embryonic origin first isolated from the inner cell mass (ICM). Two distinguishing features of ES cells are their ability to be maintained indefinitely in an undifferentiated state and their potential to develop into any cell within the body. Based on previous methods developed for mouse ES cells, human ES cell lines were first established by Dr. James Thomson and colleagues. Like mouse ES cells, human ES cells express high levels of membrane alkaline phosphatase (AP) and Oct-4, a transcriptional factor critical to ICM and germline formation. However, unlike mouse ES cells, hES cells do not express stage-specific embryonic antigen (SSEA-1). In addition, prolonged propagation of hES cells is typically achieved by coculture with primary mouse embryonic fibroblasts (MEFs) serving as feeder cells. Human ES cell lines are not able to maintain their undifferentiated state in the absence of supporting feeder layer cells, even when exogenous cytokines such as leukemia inhibitory factor (LIF) and gelatin-coated plates are used. Although stem cells from different origins require different growth conditions for self-renewal and display different cell surface markers (see Table 1), AP is the most widely used stem cell marker. The StemTAG Alkaline Phosphatase Staining and Activity Assay Kit (Fluorometric) provides an efficient system for monitoring ES cell undifferentiation/ differentiation through AP activity by both immunocytochemistry staining and quantitative activity assay. The fluorometric alkaline phosphatase activity assay is 50 times more sensitive than that obtained with the chromogenic substrate (see CBA-301).

horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions