|The Human STC1 stc1 (Catalog #MBS8248406) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS8248406 ELISA Kit recognizes Human (General) STC1.
The STC1 stc1 product has the following accession number(s) (GI #4507265) (NCBI Accession #NP_003146.1) (Uniprot Accession #P52823). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Stanniocalcin 1 ELISA Kit with the following immunoassay(s):
Typical Standard Curve/Testing Data
Principle of the Assay: The Human Stanniocalcin 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Stanniocalcin 1 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human Stanniocalcin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Stanniocalcin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Stanniocalcin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Stanniocalcin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Stanniocalcin-1 is a glycoprotein which is encoded by the STC1 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. Human Stanniocalcin-1 is a putative molecular biomarker of leukemic microenvironment and the only molecular function known up to date is a SUMO E3 ligase activity in the SUMOylation cycle. STC1 interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and dot-like fashion in the nucleus. The N-terminal region of STC1 is the function region which is responsible to establish the interaction with its partners, including SUMO1. Low resolution studies shows that STC1 is an anti-parallel homodimer in solution and the cystein 202 is responsible for the dimerization of this protein. All the 5 dissulfide bonds of human STC1 are conserved and have the same profile of fish STC.
Samples: Serum, plasma
Sensitivity: Typically less than 10 pg/ml. Human Stanniocalcin 1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.