Sphingomyelinase assay kit product blog
Tags: Assay Kit; Sphingomyelinase; Sphingomyelinase assay kit;
The Sphingomyelinase n/a (Catalog #MBS841499) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.Measurement of SMase activity in various tissues/cells extracts
Screening of SMase inhibitors. Researchers should empirically determine the suitability of the Sphingomyelinase n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the Sphingomyelinase Activity Colorimetric Assay Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Product Information: Sphingomyelinase Activity Colorimetric Assay Kit: Simple & convenient assay to measure sphingomyleinase activity in tissue samples such as brain & adhesion or suspension cells. Detection limit: < 1.25 mU/ml (~5 uU/mg tissue/cells). 100 assay
Background: Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphocholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger that induces a variety of cell regulatory phenomena such as apoptosis, cell differentiation, cell proliferation, and sterol homeostasis. There are five distinct types of SMases. MyBioSource\'s SMase Activity Assay Kit provides a simple and sensitive method for measuring neutral-SMase enzymatic activity using colorimetry (OD 570 nm). Neutral sphingomyelinase (N-SMase) is a Mg2+ sensitive enzyme that can be activated by a host of physiologically relevant and structurally diverse molecules. In this assay, SMase converts sphingomyelin substrate to phosphocholine and ceramide, which is then further utilized to produce an intermediate that reacts with a highly specific probe to generate color. This high-throughput adaptable assay kit can detect sphingomyelinase activity as low as 1.25 mU/ml (~5 uU/mg tissue/cells) in a variety of samples.
Summary: Detection method- Absorbance (OD 570 nm)
Applications- - Measurement of SMase activity in various tissues/cells extracts - Screening of SMase inhibitors.
Samples: Animal tissues: brain, heart, kidney, etc.
Cell culture: Adherent or suspension cells. Detection Method: Absorbance (OD 570 nm)
Features & Benefits: Simple, rapid & convenient
The assay can detect sphingomyleinase activity as low as 1.25 mU/ml (~5 uU/mg tissue/cells) in a variety of samples.