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SPACA3 elisa kit product blog

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Posted on 2017-05-10 14:08:06 by mybiosource_staff
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Tags: ELISA Kit; Human ELISA Kit; SPACA3; sperm acrosome associated 3; SPACA3 elisa kit;
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The Human SPACA3 spaca3 (Catalog #MBS939235) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS939235 ELISA Kit recognizes Human (General) SPACA3.

The SPACA3 spaca3 product has the following accession number(s) (GI #27777653) (NCBI Accession #NP_776246.1) (Uniprot Accession #Q8IXA5). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Sperm acrosome membrane-associated protein 3, SPACA3 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) SPACA3.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for SPACA3 and Horseradish Peroxidase (HRP) conjugated SPACA3. The competitive inhibition reaction is launched between with HRP labeled SPACA3 and unlabeled SPACA3 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of SPACA3 in the sample. The color development is stopped and the intensity of the color is measured.

Samples: Serum, plasma, tissue homogenates
Assay Type: Competitive
Detection Range: 6 pg/ml-1200 pg/ml
Sensitivity: 3 pg/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Human Sperm acrosome membrane-associated protein 3, SPACA3 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Vascular Diseases are some of the diseases may be linked to Human Sperm acrosome membrane-associated protein 3, SPACA3 ELISA Kit. Testis tissues are correlated with this protein.
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