Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow SOD4 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • MLL2 elisa kit blog
 • XIAP elisa kit blog
 • N-MID-OT elisa kit blog
 • PRX elisa kit blog
 • NQO1 elisa kit blog
 • NEFL elisa kit blog
 • LCA5 elisa kit blog
 • AQP-5 elisa kit blog
 • FBXO32 elisa kit blog
 • sP53 elisa kit blog
 • OTOG elisa kit blog
 • ENSalpha elisa kit blog
 • ROCK1 elisa kit blog
 • ST6GALNAC2 elisa kit blog
 • PC/CPG elisa kit blog
 • CX3XR1 elisa kit blog
 • HBXIP elisa kit blog
 • N-ACHTR elisa kit blog
 • SELE elisa kit blog
 • CTSL elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Epiregulin (EREG) Recombinant Protein
 • Activin A (INHBA) Recombinant Protein
 • IFN GAMMA (Ifng) Recombinant Protein
 • Lambda Light Chain (IGL) Antibody
 • Anthrax Protective Antigen IgG (APA IgG) ELISA Kit
 • Prostaglandin E1 (PGE1) ELISA Kit
 • FBL Antibody
 • M-CSF Antibody
 • A Disintegrin and Metalloprotease 2 (ADAM2) ELISA Kit
 • Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) Recombinant Protein
 • Tumor necrosis factor receptor superfamily member 12A protei... Recombinant Protein
 • ADAM metallopeptidase with thrombospondin type 1 motif, 20 (... ELISA Kit
 • HPGD Antibody
 • Annexin A1 (ANXA1) Antibody
 downarrow more ...
separator

SOD4 elisa kit product blog

separator
Posted on 2017-06-17 17:21:11 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Rat ELISA Kit; Superoxide Dismutase, Copper Chaperone; SOD4 elisa kit; SOD4;
separator
The Rat SOD4 n/a (Catalog #MBS729445) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS729445 ELISA Kit recognizes Rat (Rattus, General) SOD4.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Superoxide Dismutase, Copper Chaperone ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) SOD4.

Principle of the Assay: SOD4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SOD4 antibody and an SOD4-HRP conjugate. The assay sample and buffer are incubated together with SOD4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SOD4 concentration since SOD4 from samples and SOD4-HRP conjugate compete for the anti-SOD4 antibody binding site. Since the number of sites is limited, as more sites are occupied by SOD4 from the sample, fewer sites are left to bind SOD4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SOD4 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 0.5-10ng/mL
Sensitivity: 0.1ng/ml. Intended Uses: This SOD4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat SOD4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Rat Superoxide Dismutase, Copper Chaperone ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions