SMPD1 assay kit product blog
Tags: Acid Sphingomyelinase; SMPD1; Assay Kit; SMPD1 assay kit;
The SMPD1 smpd1 (Catalog #MBS841772) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.Measurement of ASMase activity in various tissues/cell extracts
Screening of ASMase inhibitors. Researchers should empirically determine the suitability of the SMPD1 smpd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SMPD1 smpd1 product has the following accession number(s) (GI #179095) (NCBI Accession #AAA58377.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the Acid Sphingomyelinase Activity Colorimetric Assay Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Background/Introduction: Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type An and B. Acid Sphingomyelinase Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0. Subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of sample types.
SMPD1 also interacts with the following gene(s): ASAH1, ASAH2, CERK, CERS5, GBA2, SMPD2, TNFRSF1A, UGCG. The following patways have been known to be associated with this gene. Atherosclerosis, Brain Diseases, Cardiovascular Diseases, Disease Models, Animal, Inflammation, Liver Diseases, Necrosis, Nervous System Diseases, Niemann-Pick Diseases, Vascular Diseases are some of the diseases may be linked to Acid Sphingomyelinase Activity Colorimetric Assay Kit. Blood, Bone, Brain, Heart, Kidney, Liver, Lung, Muscle, Skin, Vascular tissues are correlated with this protein.