sirt2 elisa kit product blog
Tags: ELISA Kit; SIRT2; SIRT2 elisa kit; Sirtuin 2;
The sirt2 n/a (Catalog #MBS843473) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase.The sirt2 n/a product has the following accession number(s) (GI #148227874) (NCBI Accession #NP_001088636.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Sirtuin 2 (human intracellular) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Product Information: Sirtuin 2 (human intracellular) ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Human Sirtuin 2 in cells. Assay Range 0.125 - 8 ng/ml and Detection limit 80 pg/ml.100 Assays.
Background: Sirtuin 2 is a NAD-dependent histone deacetylase (39.5 kD) largely localized at the cytoplasm where it is physically associated with microtubules. SIRT2 was found to deacetylate lys40 of alpha-tubulin both in vitro and in vivo and colocalized and interacted in vivo with HDAC6, another tubulin deacetylase. It has been also shown that Sirtuin 2 is a regulator of mitotic progression that acts downstream from CDC14B in a pathway regulating mitotic exit or subsequent cytokinesis. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human Sirtuin 2 in cells. A monoclonal antibody specific for Sirtuin 2 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Sirtuin 2 is recognized by the addition of a purified polyclonal antibody specific for Sirtuin 2 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3\',5,5\'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of Sirtuin 2 in the samples. This ELISA is specific for the measurement of natural and recombinant human Sirtuin 2. It does not cross-react with human Sirtuin 1, human Sirtuin 5, human Sirtuin 6, human adiponectin, human resistin, human RBP4, human vaspin, human progranulin, human GPX3, human FTO, human Nampt, human leptin, mouse FTO, mouse Nampt. The assay range is 0.125 - 8 ng Sirtuin 2/ml and a detection limit of 80 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Applications- This assay is to be used for the in vitro quantitative determination of human Sirtuin 2 in the range of 0.125 - 8 ng Sirtuin 2/ml and a detection limit of 80 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).