|The Human SIGLEC1 siglec1 (Catalog #MBS177317) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS177317 ELISA Kit recognizes Human (General) SIGLEC1.
The SIGLEC1 siglec1 product has the following accession number(s) (GI #18202745) (NCBI Accession #Q9BZZ2.2) (Uniprot Accession #Q9BZZ2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human CD169/SIGLEC-1 PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) (Human CD169/SIGLEC-1 PicoKine ELISA Kit standard curve )
Principle of the assay: human CD169 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD169 has been precoated onto 96-well plates. Standards(NSO, S20-Q1641) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD169 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD169 amount of sample captured in plate.
Background: SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily(IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate \'flypaper\' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.
Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type: Sandwich
Detection Range: 156pg/ml-10,000pg/ml
Sensitivity: <10pg/ml. Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Human CD169/SIGLEC-1 PicoKine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. SIGLEC1 also interacts with the following gene(s): MUC1, RSAD2, SPN. The following patways have been known to be associated with this gene. Blood, Bone, Brain, Eye, Kidney, Liver, Lung, Ovary, Spleen, Uterus tissues are correlated with this protein. Atherosclerosis, Bone Marrow Diseases, Carcinoma, Cardiovascular Diseases, Disease Models, Animal, Inflammation, Lung Diseases, Necrosis, Nervous System Diseases, Skin Diseases are some of the diseases may be linked to Human CD169/SIGLEC-1 PicoKine ELISA Kit.