SIGLEC1 elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; SIGLEC1 elisa kit; SIGLEC1; CD169/SIGLEC-1;
The Mouse SIGLEC1 siglec1 (Catalog #MBS177150) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS177150 ELISA Kit recognizes Mouse SIGLEC1.The SIGLEC1 siglec1 product has the following accession number(s) (GI #347595712) (NCBI Accession #Q62230.2) (Uniprot Accession #Q62230). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse CD169/SIGLEC-1 PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The Picokine� Mouse Siglec1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Siglec1 with a 96-well strip plate that is pre-coated with antibody specific for Siglec1. The detection antibody is a biotinylated antibody specific for Siglec1. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Siglec1 with immunogen: Expression system for standard: NSO; Immunogen sequence: T20-R1639. The kit is analytically validated with ready to use reagents. To measure Mouse Siglec1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or BS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Siglec1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells\' readings against the standard curve to determine the concentration of Mouse Siglec1 in the sample.
Background/Introduction: SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily(IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate \'flypaper\' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.
Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
Detection Range: 156pg/ml-10,000pg/ml
Sensitivity: <10pg/ml.