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sICAM-1 elisa kit product blog

Posted on 2018-04-26 16:04:28 by mybiosource_staff
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Tags: ELISA Kit; Hamster ELISA Kit; Soluble Intercellular Adhesion Molecule-1; sICAM-1; sICAM-1 elisa kit;
The Hamster sICAM-1 n/a (Catalog #MBS281979) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS281979 ELISA Kit recognizes Hamster (Chinese Hamster, CHO, General) sICAM-1.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate sICAM-1 in samples. An antibody specific for sICAM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anysICAM-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sICAM-1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sICAM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

sICAM-1 n/a

Samples: Serum, Plasma, Other biological fluids
Assay Type: Sandwich. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength: 450 nm. Hamster Soluble Intercellular Adhesion Molecule-1 (sICAM-1) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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