SFT elisa kit product blog
Tags: ELISA Kit; Rat ELISA Kit; Sulfatide; SFT elisa kit; SFT;
The Rat SFT n/a (Catalog #MBS723317) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS723317 ELISA Kit recognizes Rat SFT.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Sulfatide ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve
Intended Uses: This SFA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat SFA. This ELISA kit for research use only!
Principle of the Assay: SFA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SFA antibody and an SFA-HRP conjugate. The assay sample and buffer are incubated together with SFA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SFA concentration since SFA from samples and SFA-HRP conjugate compete for the anti-SFA antibody binding site. Since the number of sites is limited, as more sites are occupied by SFA from the sample, fewer sites are left to bind SFA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SFA concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 5.0-100ng/mL
Sensitivity: 1.0ng/ml. Rat Sulfatide ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.