Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow SFRP5 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • TIMP-1 elisa kit blog
 • INS elisa kit blog
 • PIM3 elisa kit blog
 • AD elisa kit blog
 • HSP90aB1 elisa kit blog
 • HNF1B elisa kit blog
 • VCAM-1 elisa kit blog
 • F-H5N1-HA elisa kit blog
 • GALNT13 elisa kit blog
 • CDCA3 elisa kit blog
 • ADRalpha1A elisa kit blog
 • anti ryanodine antibody elisa kit blog
 • GRB10 elisa kit blog
 • PLG elisa kit blog
 • EEF1G elisa kit blog
 • C6orf115 elisa kit blog
 • FASN elisa kit blog
 • GNG3 elisa kit blog
 • IL-4 elisa kit blog
 • LUM elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Luteinizing Hormone Beta (LHb) Recombinant Protein
 • CD134L-OX40L Antibody
 • CFI ELISA Kit
 • Connective Tissue Growth Factor (CTGF) ELISA Kit
 • ATIII - Antithrombin (Serpinc1) Antibody
 • A Disintegrin and Metalloprotease 18 (ADAM18) ELISA Kit
 • CD83 Antibody
 • LRRK2 Antibody
 • Glycated hemoglobin A1c (GHbA1c) ELISA Kit
 • Phospho-JAK1 Positive Control
 • Ebola virus Antibody
 • Surfactant Associated Protein A (SP-A) ELISA Kit
 • Dengue NS1 Antibody
 • Interleukin-6 (IL-6) ELISA Kit
 downarrow more ...
separator

SFRP5 elisa kit product blog

separator
Posted on 2017-03-04 02:21:53 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Human ELISA Kit; SFRP5 elisa kit; SFRP5; Secreted Frizzled Related Protein 5 (SFRP5);
separator
The Human SFRP5 sfrp5 (Catalog #MBS450708) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS450708 ELISA Kit recognizes Human (General) SFRP5.

The SFRP5 sfrp5 product has the following accession number(s) (GI #188528609) (NCBI Accession #NP_003006.2) (Uniprot Accession #Q5T4F7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Secreted Frizzled Related Protein 5 (SFRP5) ELISA Kit with the following immunoassay(s):
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data SFRP5.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SFRP5 in human serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SFRP5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SFRP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SFRP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SFRP5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Samples: Serum, plasma and other biological fluids.
Assay Type: Sandwich
Detection Range: 1.56-100ng/mL
Sensitivity: 0.60ng/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SFRP5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SFRP5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Human Secreted Frizzled Related Protein 5 (SFRP5) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Brain, Eye, Intestine, Pancreas tissues are correlated with this protein. The following patways have been known to be associated with this gene. SFRP5 also interacts with the following gene(s): LHX5, WNT10B, WNT11, WNT2B, WNT3A, WNT4, WNT5A, WNT8A. Breast Neoplasms, Carcinoma, Carcinoma, Hepatocellular, Cardiovascular Diseases, Colonic Neoplasms, Colorectal Neoplasms, Diabetes Mellitus, Type 2, Fatty Liver, Inflammation, Necrosis are some of the diseases may be linked to Human Secreted Frizzled Related Protein 5 (SFRP5) ELISA Kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions