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SEPP1 elisa kit product blog

Posted on 2015-08-01 07:57:55 by mybiosource_staff
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Tags: ELISA Kit; Bovine ELISA Kit; SEPP1 elisa kit; SEPP1; selenoprotein P, plasma, 1;
The Bovine SEPP1 sepp1 (Catalog #MBS931484) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS931484 ELISA Kit recognizes Bovine (Cow, Cattle, General) SEPP1.

The SEPP1 sepp1 product has the following accession number(s) (GI #156631001) (NCBI Accession #NP_776884.2) (Uniprot Accession #P49907). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Bovine Selenoprotein P, SEPP1 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) SEPP1.

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SEPP1. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated SEPP1. The competitive inhibition reaction is launched between with HRP-conjugated SEPP1 and SEPP1 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of SEPP1 in the sample. The color development is stopped and the intensity of the color is measured.

Samples: Serum, plasma, tissue homogenates
Assay Type: Competitive
Detection Range: 10 ng/ml -160 ng/ml.
Sensitivity: The minimum detectable dose of bovine SEPP1 is typically less than 5 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest bovine SEPP1 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Bovine Selenoprotein P, SEPP1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Adipose Tissue, Brain, Cartilage, Embryonic Tissue, Kidney, Liver, Lung, Muscle, Skin, Testis tissues are correlated with this protein. Adenocarcinoma, Carcinoma, Infertility, Male, Inflammation, Insulin Resistance, Kidney Diseases, Liver Diseases, Nervous System Diseases, Neurodegenerative Diseases, Prostatic Neoplasms are some of the diseases may be linked to Bovine Selenoprotein P, SEPP1 ELISA Kit.
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