|The Human SEMA3F sema3f (Catalog #MBS454602) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS454602 ELISA Kit recognizes Human (General) SEMA3F.
The SEMA3F sema3f product has the following accession number(s) (GI #974005318) (NCBI Accession #NP_001305729.1) (Uniprot Accession #Q13275). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Semaphorin 3F (SEMA3F) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SEMA3F in human serum, plasma, tissue homogenates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SEMA3F. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SEMA3F. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SEMA3F, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SEMA3F in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Serum, plasma, tissue homogenates or other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 46.875-3000pg/mL
Sensitivity: <19.25pg/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SEMA3F were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SEMA3F were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Human Semaphorin 3F (SEMA3F) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Adenocarcinoma, Breast Neoplasms, Cardiovascular Diseases, Disease Models, Animal, Heart Diseases, Inflammation, Liver Neoplasms, Lung Diseases, Lung Neoplasms, Nerve Degeneration are some of the diseases may be linked to Human Semaphorin 3F (SEMA3F) ELISA Kit. Bone, Brain, Embryonic Tissue, Eye, Heart, Intestine, Kidney, Lung, Mouth, Ovary tissues are correlated with this protein. The following patways have been known to be associated with this gene. SEMA3F also interacts with the following gene(s): NRP1, NRP2, PLXNA1, PLXNA3, PLXNA4, PLXND1, VEGFA, ZEB1.